ABSTRACT
OBJECTIVE:To optimize supercritical CO2 fluid extraction (SFE-CO2) technology of volatile oil from Hui medi-cine Huihui Naogengtong for stroke,and to analyze and identify its component. METHODS:With yield of volatile oil and the con-tent of cinnamaldehydeas indexes,and extraction pressure,temperature and time as factors,single factor test and orthogonal test were designed to optimize SFE-CO2 technology;optimized technology was also validated. Physical and chemical indexes of volatile oil were determined,and the constituents of volatile oil were analyzed by GC-MS and retention index. Relative percentage contents of each component were calculated by area normalization method. RESULTS:Optimal technology was as follows as extraction pres-sure 30 MPa,extraction temperature 50 ℃ and extraction time 100 min. In verification test,the yield of volatile oil was 4.8%(RSD=0.02%,n=3),and the content of cinnamylaldehyde was 2.0 mg/g (RSD=1.8%,n=3). Physicochemical properties of volatile oil were as follows as relative density 1.05,refractive index 1.52,acid value 28.128 mg(KOH)/g,iodine value 74.47 g(I)/100 g,saponification value 54.2 mg(KOH)/g,peroxide 19.42 meq/kg. GC-MS analysis showed the similarity with retention index in literatures of 31 compounds were more than 80%,21 of them were identified and accounted for 44% of total volatile oil, among which E-cinnamaldehyde (9.14%),β-caryophyllene (3.21%),α-bisabolol (3.11%) and β-bisabolene (2.56%) had high relative percentage content. CONCLUSIONS:Optimal extraction technology is stable and feasible. The results of extraction technol-ogy and component analysis can provide important trail base and reference data for the exploration and utilization of Huihui Nao-gengtong.